Analysis Note

ControlPOSITIVE CONTROL: Lymph node (lymphocytes), skin (keratinocytes), or tonsil.

Application

Immunohistochemistry: 1 µg/mL; acetone fixed cryostat sections; 2 µg/mL following heat-induced epitope retrieval in 0.01 M citrate buffer, pH 6.0 (Banfalvi, 1999).
Immunofluorescence
Flow Cytometry: 1:20 as determined on U937 cells
Optimal working dilutions must be determined by end user.

This Anti-H-CAM Antibody, Pan, clone SFF-2 is validated for use in FC, IC, IH, IH(P) for the detection of H-CAM.

Research CategoryCell Structure

Research Sub CategoryAdhesion (CAMs)

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Immunogen

Epitope: Pan

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Linkage

Replaces: 04-1123

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Liquid in PBS.

Format: Purified

Specificity

Specific for human standard CD44. Does not distinguish between splice variants (exons v3v10).

Storage and Stability

Prior to opening maintain at -20°C until expiration date. After opening maintain frozen at -20°C in undiluted aliquots until expiration date. Avoid repeated freeze/thaw cycles.